84-3364-30　Calnexin (CANX, FLJ26570, IP90, Major Histocompatibility Complex Class I Antigen-binding Protein p88, p90, P90) (HRP) 100ul　217242-HRP

特徴

• Calnexin is a Ca++ binding protein found in ER transmembranes that binds to specific n-linked oligosaccharides found on newly synthesized proteins.
• Calnexin associates with the ER membrane phosphoglycoprotein, signal sequence receptor SSRu, and has been identified as the ER protein associated with major histocompatibility complex (MHC) class I heavy chain and with the T-cell receptor and membrane immunoglobulin before assembly of these multimeric complexes.
• Hence, calnexin may represent a molecular chaperone in the ER membrane that is selective for incompletely folded oligomeric membrane proteins newly translocated to the ER.
• Applications:Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:10,000Immunohistochemistry (Formalin fixed paraffin embedded): 1:1000-1:2000Immunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
• Control:Human brain tissue lysate
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.