RAB35 is a member of the Rab GTPases identified in the human genome.
It is ubiquitously expressed and regulates a fast endocytotic recycling events associated with the terminal steps of cytokinesis, the formation of immunological synapse, and peptide loading onto major histocompatibility complex III molecules.
RAB35 also plays a significant role in the formation of the immunological synapse (IS) between peptide-loaded antigen presenting cells (APCs) and T cells.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 1:500Optimal dilutions to be determined by the researcher.
Positive Control:Human brain tissue lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human RAB35. Species Crossreactivity: rat. Species sequence homology: canine, equine (100%).
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic linear peptide corresponding to C-terminal of human RAB35 (KLH).
