Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion.
Antibody Anti-neurexin 3 alpha was made against a peptide immunogen corresponding to a stretch of aa’s in the extracellular domain of human neurexin 3 alpha.
Although the theoretical full length of Neurexin 1alpha is 180kD, the protein detected in human brain lysates may show a band around 180kD and additional bands around 40-55kD as a possible result of the posttranslational processing previously reported.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: Requires antigen retrieval using heat treatment prior to staining paraffin sections.
Sodium citrate buffer, pH 6.0 is recommended
Optimal dilutions to be determined by the researcher.
Positive Control:PC12 cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Neurexin3. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH-conjugated linear peptide corresponding to a stretch of amino acids in the extracellular domain of human Neurexin 3
