84-3364-06　Neuronal Marker, Pan (HRP) 100ul　029557-HRP

特徴

• Antibodies to neuronal proteins have become critical tools for identifying neurons and discerning morphological characteristics in culture and complex tissue.
• While the labeling from classic histological techniques such as Golgi staining and modern molecular approaches such as GFP constructs yield excellent cytoarchitectural detail, these approaches are technically challenging and impractical for many neuroscience research needs.
• Neuron-specific antibodies are convenient precision tools useful in revealing cytoarchitecture, but are limited to the protein target distribution within the neuron, which may differ greatly from nucleus to soma to dendrite and axon.
• To achieve as complete a morphological staining as possible across all parts of neurons, this polyclonal antibody blend reacts against key somatic, nuclear, dendritic, and axonal proteins distributed across the pan-neuronal architecture that can then be detected by a single secondary antibody.
• This antibody cocktail has been validated in diverse methods, cell culture and immuno-histochemistry, giving researchers a convenient and specific qualitative and quantitative tool for studying neuronal morphology.
• Applications:Suitable for use in Immunofluorescence, Immunohistochemistry and Immunocytochemistry.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Positive Control:Rat E18 cortex primary neuron cell culture or mouse adult brain cryosections.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.