84-3363-91　INF2 (Inverted Formin-2, HBEBP2-binding Protein C, C14orf151, C14orf173) (HRP) 100ul　029515-HRP

特徴

• NF2 (inverted formin 2) is a mouse formin that has been found to interact with actin through the C-terminus to the formin homology 2 domain.
• The WASP homology 2 motif activates another region that appropriates actin monomers.
• INF2 has a unique ability to speed up polymerization and depolymerization of actin filaments.
• Mutations in the N-terminal diaphanous inhibitory domain of INF2 have been proven to be a main cause of familial focal segmental glomerulosclerosis.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:Immunohistochemistry: Requires antigen retrieval using heat treatment prior to staining paraffin sections.
• Sodium citrate buffer, pH 6.0 is recommended
• Optimal dilutions to be determined by the researcher.
• Positive Control:HeLa cell lysate
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.