Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling.
FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
FAK regulation includes phosphorylation at multiple tyrosine and serine residues.
Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase).
In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.
FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth.
Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential.
A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.
Applications:Suitable for use in Western Blot and Cell Function Assay.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Untransfected and Src-transfected RAT2 cell lysates
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes rat FAK phosphorylated at Tyr397. Species sequence homology: human, xenopus, chicken, mouse (100%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic linear peptide corresponding to human FAK phosphorylated at Tyr397 (KLH).
