Bystin and tastin serve as cytoplasmic proteins that are required for trophinin to mediate cell-to-cell adhesion between trophoblastic and endometrial epithelial cells in human placenta.
Although the trophinin gene is unique to mammals, the bystin gene (BYSL) is conserved across eukaryotes.
It is shown that bystin influences the transition from silent trophectoderm to an active trophoblast through trophinin-mediated cell adhesion.
It is also essential for embryonic stem cell survival and trophectoderm development in mouse.
Bystin is demonstrated to play a key role in ribosomal biogenesis by processing 18S RNA in the 40S subunit in human cancer cells and mouse embryos.
It is also reported that c-MYC targets BYSL, which corresponds with bystin-mediated, rapid protein synthesis, and subsequent cellular growth.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry (Paraffin): 1:300; Detects Bystin in the syncytiotrophoblastic layer of the chronic villi in human placenta
Optimal dilutions to be determined by the researcher.
Positive Control:HeLa cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Bystin, Mr ~55kD. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated linear peptide corresponding to the C-terminus of human Bystin (Accession# NP_004044). Species Sequence Homology: canine, rhesus macaque, equine, rat, bovine.
