Human Aromatase is a membrane-bound enzyme that is localized on the endoplasmic reticulum.
While it is found in gonads and adrenal glands where it converts androgens into estrogens in a classical endocrine manner, it is also expressed in various tissues where it regulates local estrogen levels or estrogen/androgen ratios in an intracrine manner.
Human Aromatase is known to have two major forms, characterized by one amino acid difference at amino acid position 264 [arginine (264R) and cysteine (264C)].
Abnormal Aromatase hyperactivity is a known cause for Aromatase excess syndrome (AES), which is characterized by excess levels of estrogen, heterosexual precocity in adolescent males and isosexual precocity in adolescent females.
On the other hand, Aromatase deficiency (AROD) in Aromatase-deficient (ArKO) mice leads to increased adiposity, an inability to synthesize estrogen, birth defects, and genital-related developmental disorders.
It is also reported to play a role in a complex immune-neuro-endocrine network management system in murine cysticercosis.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.5ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Human placenta tissue lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Aromatase.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic linear peptide corresponding to human Aromatase (KLH). Species sequence homology: Bovine, chimpanzee, rhesus macaque, porcine and canine (100%), rat and mouse (94%).
