The p32 protein is a doughnut-shaped trimer that is primarily localized in the mitochondria.
It has also been reported to be present at the cell surface and in the nucleus.
The role of p32 in mammalian cells is unclear.
The yeast p32 homologue has been reported to regulate oxidative phosphorylation, and a link to autophagy has been proposed.
Elevated expression of p32 in cancer has also been noted.
Additionally, p32 is primarily localized in hypoxic/nutrient-deprived regions within tumors and that, in addition to tumor cells, a tumor-associated macrophage/myeloid cell subpopulation closely linked to tumor lymphatics expresses high levels of p32.
The expression of p32 at the cell surface, where it can be targeted with p32-binding antibodies and peptides, adds a second level of tumor specificity to p32 expression.
The unique expression pattern of p32 in tumor cells, tumor lymphatics, and tumor-associated macrophages/myeloid cells makes p32 a potential target for the diagnosis and therapy of cancer.
Applications: Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 5ug/mlImmunocytochemistry: 1:500Optimal dilutions to be determined by the researcher.
Positive Control:A431 cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human p32. Species Crossreactivity: mouse, rat.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Cocktail of peptides corresponding to the N-terminus of human and mouse p32.
