Matrix metalloproteinases (MMPs) are a family of secreted and membrane-bound zinc endopeptidases.
Collectively, these enzymes degrade components of extracellular matrix (ECM), including collagens, fibronectin, laminin and other glycoproteins.
MMPs play an important role in wound healing, apoptosis, development, angiogenesis, cancer metastases, and tissue remodeling.
Most MMPs are secreted as inactive proproteins which are cleaved and activated; however, MT1-MMP (MMP-14) is a member of the membrane-type subfamily.
Each member of this subfamily has a transmembrane domain suggesting these proteins are expressed at the cell’s surface rather than secreted.
MT1-MMP is capable of mediating pericellular proteolysis of ECM components and thought to be an important tool for cellular remodeling of the ECM.
It is also involved in endothelial cell migration, and although not fully characterized, MT1-MMP activity appears to modulate endothelial invasion, and formation of capillary tubes during angiogenesis (Galvez, 2001).
It also appears to be a factor in monocyte recruitment during inflammation (Matias-Roman, 2005).
MT1-MMP also activates MMP-2 protein, an activity involved in tumor invasion.
Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: 1ug/mlImmunohistochemistry: 1:500Immunoprecipitation: 10ugOptimal dilutions to be determined by the researcher.
Positive Control:HT-1080 cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human MT1-MMP.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Recombinant protein corresponding to the catalytic domain of human MT1-MMP (GST-tagged). Species sequence homology: chimpanzee, equine, sheep, porcine, canine, bovine, mouse, rat, orangutan.
