Raptor is a subunit of mTORC1 which regulates cell growth and survival in response to nutrient and hormonal signals.
mTORC1 is activated in response to growth factors or amino-acids.
Within mTORC1, Raptor positively regulates mTORC1 activity and functions as a scaffold for recruiting mTORC1 substrates.
Applications:Suitable for use in Western Blot and Dot Blot
Other applications not tested.
Recommended Dilution:Dot Blot: detected Raptor in modified and non modified peptides of phosphorylated and non-phosphorylated Raptor protein.
Optimal dilutions to be determined by the researcher.
Positive Control:Modified and non modified peptides of phosphorylated and non-phosphorylated Raptor protein.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Raptor phosphorylated at Ser722.
Isotype：IgG
Calc Applications Abbrev：DB WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated linear peptide corresponding to human Raptor phosphorylated at and around Ser722. Species Sequence homology: mouse, rat, canine, bovine, goat, chicken and equine.
