Nicotinic acetylcholine receptors, or nAChRs, are cholinergic receptors that form ligand-gated ion channels in the plasma membranes of certain neurons.
nAChR is triggered by the binding of the neurotransmitter acetylcholine (ACh) and is also opened by nicotine.
After binding ACh, the nAChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
nAChRs are present in many tissues in the body: the neuronal receptors are present in the central nervous system and the peripheral nervous system; and the neuromuscular receptors are found in the neuromuscular junctions of somatic muscles.
Neuronal AChR is composed of two different types of subunits: alpha (a) and beta (b).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:10,000.
Detects Nicotinic Acetylcholine Receptor beta 4 in 10ug of rat brain lysate.
Rat brain lysate was resolved by electrophoresis, transferred to PVDF and probed with N2565-33C.
Proteins were visualized using IgG (HRP) donkey anti-rabbit and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
