84-3363-45　［取扱停止］Histone H3, phosphorylated (Ser10) (H3 Histone, Family 3A) (HRP) 100ul　H5110-14M1-HRP

特徴

• Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.
• This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4).
• The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures.
• This gene is intronless and encodes a member of the histone H3 family.
• Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element.
• This gene is found in the large histone gene cluster on chromosome 6p22-p21.3.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Immunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.
• Positive Control:Colcemid treated HeLa acid extracts
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.