84-3363-41　［取扱停止］DNA Methyltransferase 1, phosphorylated (Ser714) (Dnmt 1, MCMT, M.Hsa1, DNA cytosine 5 methyltransferase 1, DNA MTase Mmu1, DNA methyltransferase M.HsaI) (HRP) 100ul　D3931-61D-HRP

特徴

• Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development.
• Hypermethylation of CpG islands in tumor suppressor genes may be linked with development of cancer.
• Three families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3.
• Dnmt1 is constitutively expressed in proliferating cells and inactivation of Dnmt1 causes global demethylation of genomic DNA and embryonic lethality.
• Dnmt1 co-purifies with the retinoblastoma (Rb) tumor suppressor gene product, E2F1, and HDAC1.
• Dnmt1 also cooperates with Rb to repress transcription from promoters containing E2F binding sites suggesting a link between DNA methylation, histone deacetylase and sequence-specific DNA binding activity, as well as a growth-regulatory pathway that is disrupted in nearly all cancer cells.
• Phosphorylation of Ser714 is induced by EGF, but the role of this modification is unknown.
• Applications:Suitable for use in ELISA, Western Blot, and Dot Blot.
• Other applications not tested.
• Recommended Dilution:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.