Type 1 protein Ser/Thr phosphatases (PP1) have a high specific activity against phosphorylase, and are resistant to okadaic acid up to concentrations of 50 nM, but sensitive to Inhibitor-2 and microcystin LR.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:500 detected PP1 beta in 10ug of HeLa lysates, Jurkat lysates, RAW 264 lysates, HEK293 lysates, NIH/3T3 lysates, C2C12 lysates, PC12 lysates, A431 lysates and HepG2 lysates.
Immunohistochemistry (Paraffin): 1:100 detected PP1 beta in normal human testis.
Tissue was pretreated with citrate buffer, pH 6.0.
Immunoprecipitation: 10-15ug/mlOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PP1 beta at ~38kD. Does not cross react with other recombinant PP1 isoforms. Species Crossreactivity: Mouse, Rat and Bovine
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Purified peptide corresponding to aa318-327 from the C-terminus of human PP1 beta catalytic subunit, conjugated to KLH.
