PP2A is a heterotrimeric type 2 Ser/Thr phosphatase, composed of a structural subunit (A), a regulatory subunit (B), and a catalytic subunit (C).
The structural A and catalytic C subunits form the constitutive core of the enzyme, which associates with one of a large number of regulatory B subunits.
There are multiple isotypes (encoded by distinct genes) of the B subunit, grouped into three families: B/PR55, B'/PR61, and B''/PR72 .
The prototypical B (or PR55) family of subunits is encoded by three genes, Balpha, Bbeta and Bgamma .
The B' (or B56) family consists of at least seven splice variants derived from five genes.
RNA's for the two members of the B'' family, PR72 and PR130, arise by alternative splicing or use of alternate transcription start sites from the same gene.
Primary structures as well as functions of B and B' subunits are conserved from yeast to mammals.
PTPA is a member of the B' family that is required for activation of PP2A by an undefined mechanism.
PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and its high sensitivity to Okadaic Acid.
It is also thought to be the principal downmodulator of Ser/Thr kinase cascades.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 1:500Immunohistochemistry (paraffin): 1:100Optimal dilutions to be determined by the researcher.
Positive Control:NIH/3T3 Cell Lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PP2A, B beta subunit. Minimal cross reactivity against PP2A, B gamma subunit. Species Crossreactivity: mouse, rat and bovine.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide peptide conjugated to KLH corresponding to the sequence NH2-Cys-Gly-Glu-Glu-Asp-Ile-Asp-THr-Arg- Lys-Ile-Asn-Asn-Ser-Phe-COOH.
