GTP-binding proteins (G-proteins) play a critical role in signal transduction by coupling receptors to effectors.
The intracellular consequences of G-protein activation include second messenger generation, protein phosphorylation, ion channel activation, gene induction, cell growth, and differentiation.
Gi belongs to the pertussis toxin sensitive G-proteins.
Gi consists of three different subtypes of alpha-subunits (alphai1, alphai2 and alphai3).
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: Paraffin sections require HIER with citrate buffer, pH 6.0Optimal dilutions to be determined by the researcher.
Positive Control:Western Blot: L6 Cell LysateImmunohistochemistry: Rat cerebellum
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human G protein subunit alphai1 and alpha2. Species Crossreactivity: mouse, rat and bovine
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Bo Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa345-354 of Gi alpha1 and aa346-355 of Gi alpha2.
