84-3363-03　［取扱停止］Bad, phosphorylated (Ser75) (Bcl2 Antagonist of Cell Death) (HRP) 100ul　B0003-58A-HRP

特徴

• Bad is a member of the BCL-2 family.
• BCL-2 family members are known to be regulators of programmed cell death.
• This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity.
• Proapoptotic activity of this protein is regulated through its phosphorylation.
• Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin are found to be involved in the regulation of this protein.
• Bad is phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity.
• Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins.
• This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival.
• Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:250Optimal dilutions to be determined by the researcher.
• Positive Control:RAW264.7/LPS lysate.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.