Applications:Suitable for use in Western Blot and Immunoprecipitation.
Other applications not tested.
Recommended Dilution:Western Blot: A 1:500-1:2000 dilution of this lot detected Akt3/PKBg in HEK293 cell lysates
Immunoprecipitation: 5ul immunoprecipitated active Akt3/PKBg from 15nM IGF-I stimulated HEK293 cell lysates.
The kinase activity of immunoprecipitated Akt3/PKBg was determined using Akt/SGK Specific Substrate Peptide Active kinase was detected by comparing the incorporation of [32P] into Akt/SGK Substrate Peptide as catalyzed by immunoprecipitates from IGF-I stimulated (15nM) HEK293 cell lysates (1mg)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Akt3/PKBg, Mr 60kD. An additional protein was observed, Mr 50kD. Detection of both bands is inhibited by preincubation with the immunizing peptide. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to aa119-131, CSPTSQIDNIGEE of human Akt3/PKBg.
