Applications:Suitable for use in Western Blotting.
Other applications not tested.
Recommended Dilution:Western blotting: 1:200 using ECL on rat brain membranes
Optimal dilutions to be determined by the researcher.
Control Antigen Included: 40ug, lyophilized.
For negative control, preincubate 1ug of purified peptide with 1ug of antibody for one hour at RT.
Optimal concentrations must be determined by the end user.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes P2X6 Receptor. The epitope specific for the P2X6 receptor is not present in any other known proteins. Species Crossreactivity: Rat. The immunogen sequence is conserved in mouse (15/15) and human (14/17).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Rt
Immunogen：Highly purified peptide corresponding to residues 363-379 of rat P2X6 (UniProt #P51579).
