RSV is so named from the formation of syncytia, or multinucleated masses of fused cells.
Two virally specified surface glycoproteins are prominent in RSV.
The large glycoprotein is designated G and the other fusion protein is designated F.
In addition, two distinct groups may be present in RSV, Group A and/or Group B.
Applications:Suitable for use in Indirect Immunofluorescence, ELISA, Immunohistochemistry and Fusion inhibition.
Other applications not tested.
Recommended Dilutions:Indirect Immunofluorescence: 1:480Fusion inhibition: >1:256Immunohistochemistry: Paraffin sectionsOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:goat
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes all RSV antigens. Does not react with Para 1-3, Influenza A & B and Adenovirus by IFA. Non reactive with HEp-2 cells and WI-38 cells.