Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation.
Load 20ug of reduced lysate per lane for minigels.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human Kip1 p57. Species crossreactivity: Rabbit and rat.
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Rb Rt
Immunogen：An 18 amino acid synthetic peptide ([C]GSVEQTPKKPGLRRRQT) corresponding to human Kip1 p57, conjugated to KLH. The human Kip1 sequence used (amino acids 182–198) shares 13/17 amino acids with murine Kip1. No other known proteins share more than 8 amino acids with the sequence.
