Protein prune homolog, otherwise known as PRUNE or Drosophila-related expressed sequence 17, is a phosphodiesterase enzyme belonging to the PPase class C family.
PRUNE targets cAMP and cGMP second messengers and plays a role in cell proliferation and motility.
It is overexpressed in various sarcomas, including leiomyosarcomas, malignant fibrous histiocytomas, and malignant liposarcomas.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Control:HeLa S3 cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human PRUNE.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Linear peptide corresponding to human PRUNE, conjugated to KLH.
