Histone methylation occurs on arginine and lysine residues and is catalyzed by two families of proteins, the protein arginine methyltransferase family and the SET-domain-containing methyltransferase family.
Five members have been identified in the arginine methyltransferase family.
About 27 are grouped into the SET-domain family, and another 17 make up the PR domain family that is related to the SET domain family.
The retinoblastoma protein-interacting zinc finger gene RIZ1 is a tumor suppressor gene and the founding member of the PR domain family.
PRDM12 (PR domain zinc finger protein 12) also acts as a tumor suppressor and is linked to the pathogenesis of chronic myeloid leukemia.
PRDM12 is also thought to play a role in transcriptional regulation.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:Human liver tissue lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human PRDM12. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic linear peptide corresponding to human PRDM12, conjugated to KLH.
