Recommended Dilution:Immunoblot Analysis: 0.5-2.0ug/ml of this lot detected full-length ubiquitin from bovine erythrocytes, and ubiquitylated proteins in acid extracts from HeLa cells subjected to high temperature antigen retrieval
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes bovine ubiquitin, Mr ~8kD, and ubiquitinylated proteins. Species Crossreactivity: human. Broad species crossreactivity is expected based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu
Immunogen：KLH-conjugated, full-length ubiquitin isolated from bovine erythrocytes.
