Applications:Suitable for use in Western Blot, Immunoprecipitation and IP-kinase assay.
Other applications not tested.
Recommended Dilution:Western Blot: detected cdk2 in nuclear extracts from HeLa cells
Immunoprecipitation: immunoprecipitated cdk2 from 500ug of HeLa nuclear extract
Immunoprecipitation Kinase Assay: 4ug of this lot immunoprecipitated active cdk2 from HeLa nuclear extract as shown by the ability to phosphorylate Histone H1
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human p33cdk2 protein kinase, Mr 33kD. Species Crossreactivity: Human. Predicted to crossreact with mouse, rat, non-human primates and hamster.
Isotype：IgG
Calc Applications Abbrev：IP WB
Calc Crossreactivity：Hu Mk Mo Rt
Immunogen：KLH-conjugated, synthetic peptide corresponding to aa287-298 (CQDVTKPVPHLRL) of human cdk2, with a cysteine added for conjugation purposes.
