Neomycin phosphotransferase II confers resistance to kanamycin and neomycin in bacteria and G418 (Geneticin®, G418 Sulfate) in mammalian cells.
These antibiotics bind to ribosomal components and inhibit protein synthesis.
NPT II inhibits these antibiotics through phosphorylation, which is thought to interfere with their active transport into the cell.
Western Blot analysis of transfected cell lysates with anti-NPT II can be used to gauge relative transfection efficiencies in experiments where multiple transfections are performed in parallel with different plasmids.
Applications:Suitable for use in FLISA and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Western Blot: Detects NPT II in RIPA lysates from Cos-1 cells transfected with pUSE, harboring the neomycin resistance geneOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at 4°C.
Aliquots are stable at 4°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes bacterial NPT II, Mr ~30kD.
Isotype：IgG
Calc Applications Abbrev：FLISA WB
Immunogen：Full length Neomycin Phosphotransferase II (NPT II)
