Recommended Dilution:Western Blot: 1:1000 detects recombinant MKK7 1 that had been phosphorylated by MEKK1.
Recombinant MKK7 1 was left untreated or phosphorylated by MEKK1 and resolved by electrophoresis, transferred to nitrocellulose and probed with antiphospho-MKK7/SKK4 (Thr275/Ser277) (1:1000 dilution).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes MKK7/SKK4 when phosphorylated at Thr275 and Ser277, Mr 47kD. Species Crossreactivity: Human. Crossreactivity with mouse expected due to sequence homology.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to amino acids surrounding Thr275/Ser277 of human MKK7/SKK4 (KLH).
