The Low Affinity Nerve Growth Factor Receptor (also called the LNGFR or p75 neurotrophin receptor) is one of the two receptor types for the neurotrophins, a family of protein growth factors which stimulate neuronal cells to survive and differentiate.
The neurotrophins are comprised of four proteins, all of which bind to the LNGFR: Nerve Growth Factor (NGF), Brain Derived Neurotrophic Factor (BDNF), Neurotrophin-3 (NT-3), and Neurotrophin-4 (NT-4)
Nerve growth factor, the prototypical growth factor, is a protein secreted by a neuron's target.
NGF is critical for the survival and maintenance of sympathetic and sensory neurons.
NGF is released from the target cells, binds to and activates its high affinity receptor (TrkA), and is internalized into the responsive neuron.
The NGF/TrkA complex is subsequently trafficked back to the cell body.
This movement of NGF from axon tip to soma is thought to be involved in the long-distance signaling of neurons.
Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: detects p75 neurotrphin receptor in RIPA lysates from PC-12 cells.
PC-12 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with N2017-42 (0.1ug/ml).
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system
Immunoprecipitation: Reported to immunoprecipitate p75 neurotrophin receptor
Immunohistochemistry: Reported to detect p75 neurotrophin receptor in rat brain sections (3)
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes rat p75 neurotrophin receptor, Mr ~75kD.Species Crossreactivity: human and mouse
Isotype：IgG
Calc Applications Abbrev：IHC IP WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：GST fusion protein corresponding to the intracellular domain (residues 274-425) of rat p75 neurotrophin receptor.
