The protein encoded by this gene is a member of the MAP kinase family.
MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development.
This kinase is specifically activated by mitogen-activated protein kinase kinase 5 (MAP2K5/MEK5).
It is involved in the downstream signaling processes of various receptor molecules including receptor type kinases, and G protein-coupled receptors.
In response to extracelluar signals, this kinase translocates to cell nucleus, where it regulates gene expression by phosphorylating, and activating different transcription factors.
Four alternatively spliced transcript variants of this gene encoding two distinct isoforms have been reported.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Note: Basal levels of phosphorylated ERK5 in non-stimulated HeLa cells can be eliminated by treatment with Lambda Protein Phosphatase.
The calculated Molecular Weight: is 88kD but is observed at ~115kD.
Unidetified bands may appear in some lysates.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human ERK5, phosphorylated at Thr218 and Tyr220, Mr ~115kD.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Linear synthetic peptide corresponding to aa213-224 of the TXY motif, C-HQYFM[pT]E[pY]VATR, of human ERK5 phosphorylated at Thr218 and Try220, conjugated to KLH. Species Sequence Homology: bovine, mouse, rat
