Members of the glial cell line-derived neurotrophic factor (GDNF) family, including GDNF and neurturin (NTN), play key roles in the control of vertebrate neuron survival and differentiation.
A new member of the GDNF family was recently identified and designated persephin (Milbrandt et al.
1998).
Physiological responses to these neurotrophic factors require two receptor subunits, the novel glycosylphosphadidylinositol linked protein GFRa and Ret receptor tyrosine kinase GFRb (Nomenclature Committee 1997).
Following the findings of GFRa-1 and -2, a novel receptor in GFRa family was identified very recently from human and mouse and designated GFRa-3 (Jing et al.
1997; Baloh et al.
1998; Worby et al.
1998; Navelthan et al.
1998; Nomoto et al.
1998).
GFRa-3 binds persephin, thus, persephin, GFRa-3 and the Ret PTK form a complex to transduce persephin signal and to mediate persephin function.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilution determined by the researcher.
Positive Control: Mouse kidney, liver or heart tissue lysates
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Purified
Purity：Purified
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes mouse GFRa-3.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Peptide corresponding to the aa347-360 of mouse GFRa-3.
