84-3358-06　Fibronectin (FITC) 100ul　F4215-46-FITC

特徴

• Fibronectin is a high molecular weight extracellular adhesion molecule involved in many cellular processes, including tissue repair or wound healing, embryogenesis, blood clotting, and cell migration/adhesion.
• Fibronectin exists in two major forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the extracellular matrix (ECM), and 2) as a soluble disulfide-linked dimer found in the plasma.
• The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
• Fibronectin sometimes serves as a general cell adhesion molecule by anchoring cells to collagen or proteoglycan substrates.
• It can also serve to organize cellular interaction with the ECM by binding to different components of the ECM and to membrane- bound fibronectin receptors on cell surfaces.
• Applications:Suitable for use in Immunohistochemistry, RIA, FLISA, Immunofluorescence and Immunocytochemistry.
• Other applications have not been tested.
• Recommended Dilution:Immunohistochemistry: 1:80 for immunofluorescent staining of frozen mouse skin and liver tissues.
• Immunofluorescence (IC): Evaluated on NIH/3T3 cellsOptimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.