Applications:Suitable for use in Immunohistochemistry, RIA and ELISA.
Not suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 1:80.
Frozen mouse skin and liver tissuesImmunohistochemistry: Acetone or methyl-carnoy fixed, paraffin-embedded tissueRIA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes Mouse Collagen Type IV: 100%, Mouse Collagen Types I, II, III: < 0.1%, Mouse Fibronectin: < 0.1%, Mouse Laminin: < 0.01%, Human Collagen Types IV, V: < 0.1%.
Isotype：IgG
Calc Applications Abbrev：E IHC RIA
Calc Crossreactivity：Mo
Immunogen：Collagen type IV extracted and purified from mouse tumor tissues
