c-fos is a 55kD nuclear associated protein that is rapidly induced in HL60, HeLa and NIH3T3 cells by a variety of agents.
c-fos is also induced in neuronal tissue following drug induced seizures.
Applications:Suiatble for use in Western Blot and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilution:Western Blot: Detergent lysates were produced from HeLa and HL-60 cells stimulated to express c-fos protein.
Detects a band at 55-60kD on immunoblots of stimulated HeLa and HL-60 lysates.
Immunocytochemistry: Has been used in immunocytochemistry of vibratome sections of rat brains following either surgical damage to one of the hemispheres (detects c-fos and c-fos related proteins in the nuclei of neurons in the damaged hemisphere) or kainic acid treatment
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：sheep
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human c-fos. Species Crossreactivity: Rat
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide: Met-Phe-Ser-Gly-Phe-Asn-Ala-Asp-Tyr-Glu-Ala- Ser-Ser-Ser-Arg-Cys, selected from a conserved region of mouse and human c-fos.
