84-3357-73　CENP-A (Centromere Protein A) (FITC) 100ul　C2615-FITC

特徴

• The kinetophore is a macromolecular structure that is associated with the centromeres of chromosomes and is responsible for establishing and maintaining the connections with the microtubules of the mitotic spindle.
• The proteins that reside within the chromosomal domain fall into two broad classes: those that associate with the centromere throughout the cell cycle and those that are found at the centromere in a cell cycle specific manner.
• Members of the first class include CENP-A, CENP-B, and CENP-C.
• The members of the second class include several ubiquitous cellular proteins such as calmodulin, tubulin, dynein and p34 cdc2 and also CENP-E and CENP-F.
• CENP-F appears to be the earliest member to interact with the centromere-kinetochore complex.
• Applications:Suitable for use in Immunoblot anaylsis.
• Other applications have not been tested.
• Recommended Dilution:Immunoblot Analysis: 1:200-1:1000 detected CENP-A in RIPA lysates from HeLa acid extracted histones.
• 20ug control histones, were resolved by electrophoresis, transferred to nitrocellulose and probed with 2ug/ml anti-CENP-A.
• Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.