84-3357-70　STAT 2, phosphorylated (Tyr689) (Signal Transducer and Activator of Transcription 2) (FITC) 100ul　S7970-80-FITC

特徴

• STAT (for signal transducers and activators of transcription) proteins can be selectively phosphorylated on tyrosine residues by Jak family kinases and some receptors with tyrosine kinase activity.
• The STAT proteins are highly conserved in their amino termini, but have divergent carboxy termini, which are thought to be essential for their selective activity.
• Seven STAT proteins have been described (STAT1a, STAT1b, STAT2, STAT3, STAT4, STAT5, and STAT6) and range in molecular weight from 84-113kD.
• Interferon-a promotes phosphorylation of STAT1 and STAT2, which then form heterodimers that interact with the 48kD DNA binding protein ISGF3.
• This complex moves to the nucleus where it binds to interferon-stimulated response elements and activates transcription of specific genes.
• In contrast to interferon-a signaling, interferon-g only induces phosphorylation of STAT1a or b.
• STAT proteins may be involved in the intracellular signaling of many cytokines and growth factors via the activation of gene expression.
• Applications:Suitable for use in Western Blot and Immunoprecipitation.
• Other applications have not been tested.
• Recommended Dilutions:Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: FITC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.