Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion.
Antibody Anti-Neurexin-1-alpha was made against a peptide immunogen corresponding to a stretch of aa’s in the extracellular domain of human Neurexin-1-alpha.
Although the theoretical full length of Neurexin-1-alpha is 161kD, the protein detected in human brain lysates may show a band around 160kD and additional bands around 45kD as a possible result of the posttranslational processing previously reported.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1ug/mlImmunohistochemistry: 1:100Optimal dilutions to be determined by the researcher.
Positive Control:Human cerebellum tissue lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human Neurexin-1-alpha. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic linear peptide corresponding to human Neurexin-1-alpha at the extracellular laminin G-like 3 domain, conjugated to KLH. Species Sequence Homology: chimpanzee, Rhesus Macaque, canine, bovine, 100%
