Focal adhesion kinase (FAK) is a non receptor protein tyrosine kinase discovered as a substrate for Src and a key element in integrin signaling.
FAK plays a central role in cell spreading, differentiation, migration, cell death and acceleration of the G1 to S phase transition of the cell cycle.
FAK regulation includes phosphorylation at multiple tyrosine and serine residues.
Phosphorylation of tyrosine is generally associated with positive regulation and growth promotion; however, dephosphorylation at these sites occurs as cells enter mitosis (M-Phase).
In contrast, serine phosphorylation either remains high or is increased as cells enter mitosis and may play a role in focal adhesion disassembly.
FAK and its phosphorylation states have been implicated in cancer metastasis, tumor cell survival, and adhesion-independent growth.
Recent evidence indicates that elevation of FAK activity in human carcinoma cells is associated with increased invasive potential.
A central role in tumor formation and progression suggests that FAK is an attractive target for therapeutic intervention.
Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397.
Applications:Suitable for use in Western Blot and Cell Function Assay.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Untransfected and Src-transfected RAT2 cell lysates
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes rat FAK phosphorylated at Tyr397. Species sequence homology: human, xenopus, chicken, mouse (100%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic linear peptide corresponding to human FAK phosphorylated at Tyr397 (KLH).
