LCN2 (also known as Neurophil Gelatinase-Associated Lipocalin or NGAL) belongs to a superfamily of lipocalins, a collection of small, diversely functional, extracellular proteins.
NGAL is secreted from neutrophil granules in three different forms; a monomer of 25 kDa, a disulfide-linked homodimer at 46 kDa, and a disulfide-linked heterodimer with MMP-9 at 135-kDa.
It is widely known to induce development of kidney epithelia, to bind and traffic iron, and to act as a molecular signal under inflammatory conditions, such as Crohn’s Disease.
Multiple carcinoma studies have also shown elevated expression levels of LCN2 in several different human tumors affecting such organs as: colon, lung, breast, ovaries and pancreas.
Additionally, there has been great interest in this protein as a possible marker for: onset of Acute Kidney Injury following cardiac surgery, the progression of Chronic Kidney Disease (CKD), and the survival chances of patients with Chronic Heart Failure (CHF).
Applications: Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Westrn Blot: 5ug/mlImmunohistochemistry: 1:200Optimal dilutions to be determined by the researcher.
Positive Control:Human kidney tissue lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human LCN2.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Hu
Immunogen：Linear peptide corresponding to the mature chain of human LCN2 (KLH). Species sequence homology: mouse, bovine and rat.
