84-3356-21　ASPM (ASP (Abnormal Spindle)-like Microcephaly Associated, Microcephaly, Primary Autosomal Recessive 5, HGNC:19048, MCPH5) (Biotin) 100ul　A3883-40A-Biotin

特徴

• ASPM gene is the human ortholog of the Drosophila melanogaster 'abnormal spindle' gene (asp), which is essential for normal mitotic spindle function in embryonic neuroblasts (Bond et al., 2002 [PubMed 12355089]).
• The mouse gene ASPM is expressed specifically in the primary sites of prenatal cerebral cortical neurogenesis.
• Molecular Weight:218, 95, 75kD Observed UniProtKB/Swiss-Prot entry Q8IZT6 describes 2 isoforms produced by alternative splicing: Isoform 1 at 409.800kD and Isoform 2 at 217.794kD.
• Multiple other isoforms in ref: Human Molecular Genetics, 2005, Vol.
• 14, No.
• 15 2155-2165.
• Applications:Suitable for use in ELISA and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilutions:ELISA: Tested in direct ELISA against the ASPM immunogen
• Immunohistochemistry (Paraffin): 1:300.
• Positive and background staining patterns on formalin fixed, paraffin embedded human ductal carcinoma.
• Tissue pretreated with citrate pH 6.0 using antigen retrieval techniques.
• Detected using HRP/DAB.
• Immunoreactivity is seen in nucleus and cytoplasm
• Optimal dilutions to be determined by the researcher.
• Positive Control:Human ductal carcinoma tissue
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.