PI3 Kinase (Phosphatidylinositol 3-Kinase) is responsible for phosphorylation of the 3 position of the inositol ring of PI(4,5)P2, to generate PI(3,4,5)P3, a potent second messenger required for survival, signaling, and insulin action.
PI3 Kinase is a heterodimeric complex composed of an 85kD regulatory subunit and a 110kD catalytic subunit.
Tyrosine phosphorylation of growth factor receptors creates docking sites for binding of p85 (through its SH2 domains) on the receptors; p85 brings with it p110, which is then proximal to its phospholipid substrate on the membrane.
PI3 Kinase is also activated by Ras, and by the beta:gamma subunits of heterotrimeric G-proteins.
PI3 Kinase is inhibitable by wortmannin, a useful tool for the study of the PI3 Kinase signaling pathway.
Applications:Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 0.5ng/wellWestern Blot: 0.05-0.1ug/mlImmunocytochemistry: 1:100Optimal dilutions to be determined by the researcher.
Positive Control:HEK-293 cell lysate
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes the internal domain of human PI3 Kinase, p110 beta. Species Sequence Homology: Monkey, canine, Rat and Mouse.
Isotype：IgG
Calc Applications Abbrev：E IC IHC IP WB
Calc Crossreactivity：Hu
Immunogen：Synthetic linear peptide corresponding to the internal domain of PI3 Kinase, p110 beta (KLH).
