Recommended Dilution:Immunoblot Analysis: 0.5-2.0ug/ml of this lot detected full-length ubiquitin from bovine erythrocytes, and ubiquitylated proteins in acid extracts from HeLa cells subjected to high temperature antigen retrieval
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：bovine
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes bovine ubiquitin, Mr ~8kD, and ubiquitinylated proteins. Species Crossreactivity: human. Broad species crossreactivity is expected based on sequence homology.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu
Immunogen：KLH-conjugated, full-length ubiquitin isolated from bovine erythrocytes.
