Calmodulin (CaM), a small 17 kDa ubiquitous Ca2+-binding protein acts as an intracellular Ca2+ receptor and transduces Ca2+ transients, usually in response to growth factors.
It mediates the activity of a number of Ca2+ regulating enzymes, including protein kinases, phosphatases, nitric oxide synthases, and phosphodiesterase.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:5000.
Detects a recombinant protein containing the calmodulin binding protein epitope tag in lysates from transformed E.
coli.
Lysates from E.
coli transformed with a plasmid encoding a GST-CBP, calmodulin binding protein epitope tagged.
Proteins were resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-09.
Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes recombinant proteins containing the calmodulin binding protein epitope tag. Species sequence homology: rabbit, mouse and rat.
Isotype：IgG
Calc Applications Abbrev：WB
Immunogen：A synthetic peptide, MKRRWKKNFIAVSAANRFKKISSSGAL corresponding to the Calmodulin Binding Protein (CBP) epitope tag found in several E. coli expression vectors (KLH).
