Recommended Dilution:Western Blot: detected AMP-activated protein kinase a subunits in a rat liver protein preparation
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by Protein A affinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity:Recognizes human AMP-activated protein kinase a subunits, Mr ~63kD. An unknown protein was also detected, Mr ~44kD, that was competed with the immunizing peptide. Species Crossreactivity: rat
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu Rt
Immunogen:KLH-conjugated, synthetic peptide corresponding to aa4-22 of human AMPactivated protein a1 catalytic subunit (AEKQKHDGRVKIGHYILGD). The sequence shares 18/19 residues with rat and human AMP-activated protein kinase a2 catalytic subunit.