S-Adenosyl-I-methionine-dependent protein arginine N-methyltransferases (PRMTs) methylate arginine residues within a variety of proteins, particularly histones.
At least four distinct mammalian family members have now been described, including PRMT1, PRMT3, CARM1/PRMT4 and JBP1/PRMT5
Applications:Suitable for use in Western Blotting, ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:100-1:500.
Reacts with a band of approximately 57kD on whole HL60 cell lysate.
Additional bands may be present depending on sample used
ELISA (direct): 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified immunoglobulin
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human PRMT3 (Protein Arginine N-Methytransferase 3).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide from N-terminus of human PRMT3.
