Applications:Suitable for use in Immunohistochemistry, RIA and ELISA.
Not suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry: 1:80.
Frozen mouse skin and liver tissuesImmunohistochemistry: Acetone or methyl-carnoy fixed, paraffin-embedded tissueRIA: 1:5000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein G affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes Mouse Collagen Type IV: 100%, Mouse Collagen Types I, II, III: < 0.1%, Mouse Fibronectin: < 0.1%, Mouse Laminin: < 0.01%, Human Collagen Types IV, V: < 0.1%.
Isotype：IgG
Calc Applications Abbrev：E IHC RIA
Calc Crossreactivity：Mo
Immunogen：Collagen type IV extracted and purified from mouse tumor tissues
