Applications:Suitable for use in Immunohistochemistry, Radioimmunoassay and ELISA.
Not recommended for paraffin embedded tissue sections or Western Blots.
Other applications not tested.
Recommended Dilution:Immunohistochemistry: 1:40 for immunofluorescent staining of frozen chicken skin and liver tissues.
ELISA: ≥ 1:00–1:200Optimal dilutions to be detremined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:chicken
Grade:Purified
Purity:Purified IgG fraction
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity:Recognizes chicken Collagen Type I. Shows less than 0.1% reactivity with chicken Collagen Types III, V; Human, bovine, mouse and rat Collagen Type I; and chicken Fibronectin.
Isotype:IgG
Calc Applications Abbrev:E IHC RIA
Calc Crossreactivity:Ch
Immunogen:Collagen Type I extracted and purified from chicken skin