Cofilin 2 is a small actin-binding protein that plays a pivotal role in cytokinesis, endocytosis, embryonic development, stress response and tissue regeneration.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2.0ug/ml detects Cofilin 2 in RIPA lysates from rat L6 cell lysates.
Rat L6 lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C7506-50 (1ug/ml).
Proteins were visualized using an IgG (HRP) goat anti-rabbit and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human Cofilin 2 at ~21kD. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：Synthetic peptide corresponding to aa136-152, C-QVNGLDDIKDRSTLGEK, of human Cofilin 2 with N-terminal cysteine for KLH conjugation. Species Sequence Homology: mouse and chicken
