AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity.
AMPK exists as a heterotrimeric complex comprised of catalytic alpha subunits (62 kD) and non-catalytic beta and gamma subunits.
The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence.
AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation.
Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression.
Phosphorylation of threonine 172 in the activation loop of the alpha subunit is a key determinant of AMPK activity.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.5-2ug/ml detected AMP-activated Protein Kinase a1 in RIPA lysates from human non-stimulated A431 cells.
Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with A1475-01.
Proteins were visualized using an IgG (HRP) goat and a chemiluminescence detection system
Optimal dilutions to be determined by the researcher.
Included Positive Antigen Control:A0001-50: A431 Cells, Non-Stimulated in RIPA Buffer, Sample Buffer
Recommended Secondary Antibodies:I1904-39: IgG, X-Adsorbed (HRP) Pab Gt x RbI1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt x RbI1904-46Q: IgG, H&L (HRP) Pab Gt x Rb
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human AMPK a1, Mr 63kD. Species Crossreactivity: rat and mouse.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa376-392, C-ARHTLDELNPQKSKHQG-COOH of human AMP-activated Protein Kinase alpha1, conjugated to KLH (NP_006242).
