Applications:Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 0.5-1ug/ml detected histone H3on 10ug of Colemid treated HeLa acid extract.
Colemid treated HeLa acid was resolved by electrophoresis, transferred to PVDF and probed with H5110-14B.
Proteins were visualized using a goat anti-rabbit secondary antibody (HRP) and a chemiluminescence detection system
Immunoprecipitation: 10ug specifically immunoprecipitated phosphorylated histone H3 from HeLa acid extract and Colcemid treated HeLa acid extracted RIPA lysate
Immunocytochemistry: 1:500 detected Histone H3 in HeLa and A431 cells
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human histone H3 phosphorylated at Serine 10 at ~17kD. Species Crossreactivity: mouse. Broad species reactivity expected due to sequence homology.
Isotype：IgG
Calc Applications Abbrev：IC IP WB
Calc Crossreactivity：Dr Hu Th Xe
Immunogen：Linear peptide coresponding to human Histone H3 at Ser10
